NAPLEX (North American Pharmacist Licensure Examination) Practice Exam

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What reagent is used in the Bacterial endotoxins test (BET)?

  1. Sodium chloride

  2. Limulus amebocyte lysate (LAL)

  3. Potassium permanganate

  4. Calcium carbonate

The correct answer is: Limulus amebocyte lysate (LAL)

The correct answer is B. Limulus amebocyte lysate (LAL). The LAL reagent is derived from the blood cells of the horseshoe crab and is used in the Bacterial endotoxins test (BET) to detect the presence of endotoxins in pharmaceutical products. Endotoxins are part of the outer membrane of certain gram-negative bacteria and can cause severe reactions in humans if present in pharmaceuticals, making their detection crucial for ensuring product safety. Sodium chloride, potassium permanganate, and calcium carbonate are not used as reagents in the Bacterial endotoxins test (BET).

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